The Short Line Safety Institute (SLSI) is dedicated to the continuous improvement of safety and safety culture across all short line and regional railroads in the United States. Strengthening a railroad’s safety culture may result in less frequent or severe accidents and create a safer working environment. SLSI defines safety culture as the shared values, actions, and behaviors that demonstrate a commitment to safety over competing goals and demands, based on the U.S. Department of Transportation (DOT) Safety Council’s safety culture definition (Morrow & Coplen, 2017). SLSI conducted a study to assess the fidelity of its Safety Culture Assessment (SCA) model. This report summarizes findings from this study and discusses areas for enhancement of the assessment process.