This report documents stakeholder perspectives and opinions on the fatigue and safety of freight locomotive engineers. This work follows an online survey sponsored by the Federal Railroad Administration, developed by the Virginia Tech Transportation Institute, and distributed to members of the Brotherhood of Locomotive Engineers and Trainmen and the Sheet Metal Air Rail Transportation-Transportation Division labor unions. In this follow-on research, the team worked with labor and industry focus groups to gain a more in-depth understanding of fatigue and safety issues from a stakeholder perspective. Views on fatigue and safety concerns varied by stakeholder category. Labor stakeholders attributed fatigue to management policies and practices, including variable scheduling. Industry stakeholders described lifestyle choices and personal responsibility as being critical to fatigue management.